WI 38 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90020107, human lung (fetal), Fibroblast-like

• Sinônimo(s): WI38 Cells, Wistar Institute-38 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human lung (fetal)
• embalagem: tube of 5 μg 90020107-DNA-5UG; pkg of vial of cells 90020107-1VL
• modo de crescimento: Adherent
• cariótipo: 2n = 46, diploid except at high passage number
• morfologia: Fibroblast-like
• produtos: Collagen
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian foetal lung

Descrição de linhagem celular

From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.

Aplicação

From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.

Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x10⁴ viable cells/cm². Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO₂.

Virus studies (rhinoviruses), vaccine production

WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.

Ações bioquímicas/fisiológicas

Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.

Nota de análise

Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS

Outras notas

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