CHP-134

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6122002, human nerve (neuroblastoma), Neuronal

• Sinônimo(s): CHP134 Cells, Children′s Hospital of Philadelphia-134 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human nerve (neuroblastoma)
• embalagem: tube of 5 μg 06122002-DNA-5UG; pkg of vial of cells 06122002-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Neuronal
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human neuroblastoma tumour mass of left adrenal gland

Descrição de linhagem celular

A neuroblastoma cell line established from a tumour of the left adrenal area of an 18-month-old male patient. The patient was previously treated with vincristine, cyclophosphamide, dimethyltriazenoimidazole carboxamide, adriamycin, and irradiation. The cells grow as adherent colonies consisting of neuroblast-like cells forming dense mounds. Individual cells radiate from the mounds which reach confluency after routine passaging for several months. This cell line has been reported to have tyrosine hydroxylase activity.

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 8,11
D16S539: 11,13
D5S818: 11,12
D7S820: 9
THO1: 9,9.3
TPOX: 8
vWA: 18,19

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split confluent cultures 1:2 to 1:10 every 2-3 days, use gentle agitation to dislodge cells, trypsin/EDTA should not be necessary as cells only adhere loosely; 5% CO₂; 37°C.

Outras notas

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