MCA-RH 7777

90021504, rat liver, Hepatocyte

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: MCA-RH 7777, 90021504
• fonte biológica: rat liver
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Hepatocyte
• produtos: Alpha-foetoprotein
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat Buffalo hepatoma

Descrição de linhagem celular

Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce α-fetoprotein (AFP).

Aplicação

Tumourigenicity and oncogeny studies

Meio de cultura

Swimm′s S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. NB: Cells are shed from the monolayer before confluency is achieved.

Outras notas

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