CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• Código UNSPSC: 41106514
• fonte biológica: feline kidney
• modo de crescimento: Adherent
• cariótipo: Hypodiploid, modal no 36
• morfologia: Epithelial
• produtos: Carcinoembryonic antigen (CEA)
• receptores: Not specified

Propriedades

• Nome do produto: CRFK (BVD Ag negative), 86093002
• fonte biológica: feline kidney
• modo de crescimento: Adherent
• cariótipo: Hypodiploid, modal no 36
• morfologia: Epithelial
• produtos: Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Cat kidney

Descrição de linhagem celular

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

Aplicação

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

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