About This Item
Código UNSPSC:
12352202
NACRES:
NA.84
Produtos recomendados
Condições de expedição
dry ice
Nível de qualidade
temperatura de armazenamento
−20°C
Informações sobre genes
human ... CASP8(841)
Descrição geral
Caspases (cysteine-requiring aspartate proteases) belong to a highly conserved family of cysteine proteases with specificity for aspartic acid residues found in their substrates. Caspases play a central role in apoptosis. Inhibition of caspases prevents the cell death process. Currently, there are 13 caspases recognized in mammals. Caspases are classified as initiators and effectors. Caspase 8 belongs to the class of initiators. It possesses a prodomain at the terminal and contains the death effector domain which serves as protein-protein interaction motif for caspase 8. With respect to substrate specificity, caspase 8 is classified as a member of group III. It specifically recognizes the (I/L/V) EXD sequence on the substrate.
Aplicação
The Caspase 8 Colorimetric Assay Kit is based on the hydrolysis of the peptide substrate Acetyl-Ile-Glu-Thr-Asp-p-Nitroaniline (Ac-IETD-pNA) by caspase-8 that results in the release of a p-Nitroaniline (p-NA) moiety. The p-NA is read at 405 nm. The concentration of the p-NA released can be calculated from a calibration curve prepared from p-NA standards (included with the kit).
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
The kit detects caspase 8 activity in crude and purified preparations of caspase 8.
Complete kit contains:
- purified caspase 8 for a positive control
- caspase 8 inhibitor
- p-NA fluorescent standard
Quantidade
The kit provides sufficient reagents for 100 tests of 100 μL in 96-well plates.
Somente componentes do kit
Nº do produto
Descrição
- N-Acetyl-Ile-Glu-Thr-Asp-al, ≥97% (HPLC) 100 μL
- Acetyl-Ile-Glu-Thr-Asp-p-nitroaniline Substrate 100 μL
- 10X Assay Buffer 5 mL
- 5X Lysis Buffer 5 mL
Código de classe de armazenamento
10 - Combustible liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Gondi CS, et al.
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