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CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

Sinônimo(s):

Caspase-3 Activity Fluorometric Kit

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About This Item

Número CE:
Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

Nível de qualidade

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... CASP3(836)

Descrição geral

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Aplicação

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Definição da unidade

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Somente componentes do kit

Nº do produto
Descrição

  • Water (17-18 megaOhm) 125 mL

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS

  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS

  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

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Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3


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