APT139
Caspase 9 Colorimetric Activity Assay Kit, LEHD
Caspase-9 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the LEHD.
Sinônimo(s):
Mch-6, ICE-LAP6, Apaf-3
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About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
Produtos recomendados
Nível de qualidade
reatividade da espécie (prevista por homologia)
mammals
fabricante/nome comercial
Chemicon®
técnica(s)
activity assay: suitable
nº de adesão NCBI
nº de adesão UniProt
método de detecção
colorimetric
Condições de expedição
dry ice
Informações sobre genes
human ... CASP9(842)
Descrição geral
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.
Aplicação
Caspase-9 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the LEHD.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Componentes
5X Cell Lysis Buffer (Part No. 90065): 5 mL
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-9 Substrate (Ac-LEHD-pNA) (Part No. 90083): 250 μL of 3 mg/mL solution
Caspase-9 Inhibitor (Ac-LEHD-CHO) (Part No. 90087): 50 μL of 100 μM (0.054 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-9 Substrate (Ac-LEHD-pNA) (Part No. 90083): 250 μL of 3 mg/mL solution
Caspase-9 Inhibitor (Ac-LEHD-CHO) (Part No. 90087): 50 μL of 100 μM (0.054 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
Armazenamento e estabilidade
Store kit materials at -20°C up to their expiration date.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-9 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-9 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
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Vanessa Blas-Valdivia et al.
Frontiers in pharmacology, 12, 671614-671614 (2021-07-24)
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