A9891

7-Amino-4-methylcoumarin

chromophore for enzyme substrates, fluorogenic, ≥98% (HPLC), powder

• Sinônimo(s): Coumarin 120
• Fórmula empírica (Notação de Hill): C₁₀H₉NO₂
• Número CAS: 26093-31-2
• Peso molecular: 175.18
• Beilstein: 142231
• Número CE: 247-454-8
• Número MDL: MFCD00006868
• Código UNSPSC: 12352204
• ID de substância PubChem: 24891406
• NACRES: NA.32

Propriedades

• product name: 7-Amino-4-methylcoumarin, Chromophore for substrates
• Nível de qualidade: 200
• descrição: chromophore for enzyme substrates
• Ensaio: ≥98% (HPLC)
• forma: powder
• pf: 223-226 °C (lit.)
• solubilidade: acetone: 10 mg/mL, clear, colorless to yellow
• fluorescência: λ_ex 365 nm; λ_em 440 nm in ethanol(lit.)
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: CC1=CC(=O)Oc2cc(N)ccc12
• InChI: 1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
• chave InChI: GLNDAGDHSLMOKX-UHFFFAOYSA-N

Descrição

Aplicação

7-Amino-4-methylcoumarin has been used:

• to determine cathepsin B like- activity
• as a substrate for leucine aminopeptidase (LAP)
• in chymotryptic assay
• as a standard to detect the activation of caspase-3 during
• sanguinarine-induced damage
• protection by human DEAD-box DDX3

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.

Ações bioquímicas/fisiológicas

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

Nota de preparo

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Outras notas

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves