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Documentos Principais

C9284

Sigma-Aldrich

Catalase−Agarose

pH 5.4(isoelectric point), ammonium sulfate suspension, enzyme from bovine liver

Sinônimo(s):

H2O2:H2O2 Oxidoreductase

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About This Item

Número da licença da enzima:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

enzyme from bovine liver

descrição

optimum pH ~ 7.0

Formulário

ammonium sulfate suspension

peso molecular

tetramer ~250 kDa

concentração

≥800 units/mL

Extensão da rotulagem

60,000-120,000 units per g agarose (1900-3800 units per ml of agarose)

Matriz

4% cross-linked beaded agarose

pH

5.4(isoelectric point)

temperatura de armazenamento

2-8°C

Aplicação

Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria.
Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H2O2) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing. Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H2O2) that can degrade the target product.

Ações bioquímicas/fisiológicas

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.

Atenção

Freezing of solutions is not recommended.

Definição da unidade

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

forma física

Suspension in 3.2 M (NH4)2SO4, pH 7, containing preservative

Inibidor

Nº do produto
Descrição
Preços

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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