C2436

Cytochrome c from Saccharomyces cerevisiae

≥85% based on Mol. Wt. 12,588 basis

• Número CAS: 9007-43-6
• Número CE: 232-700-9
• Número MDL: MFCD00130890
• Código UNSPSC: 12352202
• NACRES: NA.61

Propriedades

• fonte biológica: Saccharomyces cerevisiae
• Ensaio: ≥85% based on Mol. Wt. 12,588 basis
• forma: powder
• peso molecular: 12,588 Da
• técnica(s): cell based assay: suitable
• solubilidade: water: 10 mg/mL, clear, red to red-brown (dark red)
• nº de adesão UniProt: P00044
• aplicação(ões): cell analysis
• temperatura de armazenamento: −20°C
• Informações sobre genes: Saccharomyces cerevisiae ... CYC1(853507)

Descrição

Descrição geral

Cytochrome c (Cytc) comprises 104 amino acids and is a nuclear-encoded mitochondrial protein. This peripheral membrane protein is in spherical shape and has a molecular weight of 12,000 Da.

Aplicação

Cytochrome c from Saccharomyces cerevisiae has been used in oocyte injection to induce caspase-3 activation and p38 MAPK (mitogen-activated protein kinase). It has also been used to analyze the aerobic reactions of nitric oxide.

Ações bioquímicas/fisiológicas

Cytochrome c (Cytc) participates in mitochondrial electron transport and intrinsic type II apoptosis. It plays a key role in aerobic energy production. This multi-functional enzyme serves as a single electron carrier. Cytc helps in the formation of the apoptosome.

The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. Tracking cellular localization of cytochrome c in S. cerervisiae demonstrates that mitochondria are strongly involved in programmed cell death, a process very similar to apoptosis in higher organisms.

Qualidade

Care has been taken to maintain the native form of the protein. It has not been artificially oxidized or reduced during purification. Contains variable amounts of reduced cytochrome c.

Nota de preparo

Prepared without using TCA.

Outras notas

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)