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C2037

Sigma-Aldrich

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis

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About This Item

Número CAS:
9007-43-6
Número CE:
232-700-9
Número MDL:
MFCD00130890
Código UNSPSC:
12352202
NACRES:
NA.61

fonte biológica

bovine heart

Nível de qualidade

300

Ensaio

≥95% based on Mol. Wt. 12,327 basis

Formulário

powder or crystals

peso molecular

12327 Da

técnica(s)

cell based assay: suitable

adequação

suitable for molecular biology

nº de adesão UniProt

P62894

aplicação(ões)

cell analysis

temperatura de armazenamento

−20°C

Informações sobre genes

cow ... CYC1(512500)

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Descrição geral

Cytochrome C is a heme protein. It is localized between the inner and outer mitochondrial membranes.

Aplicação

Cytochrome c from bovine heart has been used:
  • for cytochrome oxidase staining using the mice brain tissue
  • as a component of control mixture to evaluate the accuracy of tandem mass spectra in identifying peptides
  • to study its effect on the stability, transport, and retention of biochar colloids (BCs) in saturated porous media under various solution pH and ionic strength (IS) conditions
  • as a component of staining solution for the detection of cytochrome oxidase activity in the brain tissue sections of mouse
  • as a component in the assay to study its superoxide scavenging efficiency
  • as a component of serum-free DMEM media to evaluate non-specific cytochrome C reduction in astrocytes and HT-29 cells

Ações bioquímicas/fisiológicas

Cytochrome C is a key mediator of oxidative phosphorylation. It mediates the electron transport between complex III and complex IV of the respiratory chain.

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome.
The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.
The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.

Nota de preparo

Prepared using TCA.

Outras notas

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SLCF8491
STBJ0284
SLCC1127
SLBR2608V
SLBX3678

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Wen Yang et al.
Environmental pollution (Barking, Essex : 1987), 246, 855-863 (2019-01-10)
Application of biochar in the field has received considerable attention in recent years, but there is still little known about the fate and transport of biochar colloids (BCs) in the subsurface. Natural organic matter (NOM), which mainly consists of humic
Shiyu Xia et al.
Nature, 593(7860), 607-611 (2021-04-23)
As organelles of the innate immune system, inflammasomes activate caspase-1 and other inflammatory caspases that cleave gasdermin D (GSDMD). Caspase-1 also cleaves inactive precursors of the interleukin (IL)-1 family to generate mature cytokines such as IL-1β and IL-18. Cleaved GSDMD
Stephanie Moore et al.
Journal of chromatography. A, 1476, 1-8 (2016-11-24)
Despite the developments for faster liquid chromatographic and mass spectral detection techniques, the standard in-solution protein digestion for proteomic analyses has remained relatively unchanged. The typical in-solution trypsin protein digestion is usually the slowest part of the workflow, albeit one
Nicole J Edwards et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(12), 15753-15770 (2020-10-23)
Ischemia reperfusion (IR) injury results in devastating skeletal muscle fibrosis. Here, we recapitulate this injury with a mouse model of hindlimb IR injury which leads to skeletal muscle fibrosis. Injury resulted in extensive immune infiltration with robust neutrophil extracellular trap
Maria M Shchepinova et al.
Cell chemical biology, 24(10), 1285-1298 (2017-09-12)
Mitochondrial superoxide (O2⋅-) underlies much oxidative damage and redox signaling. Fluorescent probes can detect O2⋅-, but are of limited applicability in vivo, while in cells their usefulness is constrained by side reactions and DNA intercalation. To overcome these limitations, we developed
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Chromatograms

application for HPLCapplication for HPLCapplication for HPLC

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