B9277
Monoclonal Anti-Band 3 antibody produced in mouse
clone BIII-136, ascites fluid
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About This Item
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
BIII-136, monoclonal
contém
15 mM sodium azide
reatividade de espécies
human
técnica(s)
immunoprecipitation (IP): suitable using human erythrocytes
indirect immunofluorescence: suitable using methanol-fixed human erythrocytes
western blot: 1:5,000 using human erythrocytes
Isotipo
IgG2a
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... SLC4A1(6521)
Descrição geral
Band 3 is a hydrophobic protein, it exists in erythrocytes as a dimer and tetramer and has a strong tendency to aggregate because of oxidative stress.
Band 3 is an anion exchanger that is abundantly found in erythrocyte membranes. This integral membrane anion exchanger protein regulates mechanical stability and ion homeostasis across the red blood cell membrane. Genetic alterations in Band 3 have been associated with familial distal renal tubular acidosis
Monoclonal Anti-Band 3 antibody detects Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. The product specifically binds to the cytoplasmic amino-terminal protein of band 3 (the epitope is approx. 20 kD from the N-terminal end). As the epitope is not located at the erythrocyte surface, the antibody product does not agglutinate red blood cells. Furthermore, its cell surface binding cannot be detected by an indirect agglutination assay. The antibody does not localize Band 3 from horse, bovine, pig, guinea pig, dog or mouse erythrocytes, nor does it localize Band 3 from nonerythroid human fibroblast extract.
Monoclonal Anti-Band 3 antibody detects Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. The product specifically binds to the cytoplasmic amino-terminal protein of band 3 (the epitope is approx. 20 kD from the N-terminal end). As the epitope is not located at the erythrocyte surface, the antibody product does not agglutinate red blood cells. Furthermore, its cell surface binding cannot be detected by an indirect agglutination assay. The antibody does not localize Band 3 from horse, bovine, pig, guinea pig, dog or mouse erythrocytes, nor does it localize Band 3 from nonerythroid human fibroblast extract.
Especificidade
The antibody recognizes an epitope located in the cytoplasmic N-terminus of the band 3 molecule/proteins (90-100kDa).
Imunogênio
Glycophorin B from human erythrocytes.
Aplicação
Monoclonal Anti-Band 3 antibody can be used for western blot, immunoprecipitation and indirect immunofluorescence using human erythrocytes.
Monoclonal Anti-Human Band 3 has been used in immunoblotting. It may also be used in the study of red cell structures and functions and to study the fragmentation of the cytoplasmic domain of band 3 protein in vivo and in vitro.
Monoclonal anti-Band 3 antibodies can be used in ELISA and immunoprecipitation. It may also be used for immunofluorescent staining.
Ações bioquímicas/fisiológicas
Band 3, a 90-100kD protein is the major integral protein of human erythrocytes responsible for anion exchange. It also regulates the intracellular pH. Monoclonal anti-Band 3 antibody is useful in in vivo and in vitro study of fragmentation of cytoplasmic domain of band 3 protein. It may also be used for immunoblot analysis. Monoclonal Anti-Human Band 3 antibody reacts specifically with cytoplasmic N-terminal band 3 proteins (90-100kD).
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Mohammad Al-Ansari et al.
BMC hematology, 15, 17-17 (2015-12-22)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is associated with erythrocyte sensitivity to oxidative damage and hemolytic crises. In β-thalassemia major, where hemoglobin instability imposes oxidative stress, erythrocytes show reduced hENT1 nucleoside transporter expression and decreased nucleoside uptake. This study investigated hENT1 expression
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Nature communications, 9(1), 4960-4960 (2018-11-25)
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L J Bruce et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 76(5), 723-728 (1999-06-03)
In distal renal tubular acidosis (dRTA) the tubular secretion of hydrogen ion in the distal nephron is impaired, leading to the development of metabolic acidosis, frequently accompanied by hypokalemia, nephrocalcinosis, and metabolic bone disease. The condition can be familial, when
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PloS one, 11(1), e0147262-e0147262 (2016-01-28)
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Reduced PKC α Activity Induces Senescent Phenotype in Erythrocytes.
Govekar, R., B., et al.
Anemia, doi:10-doi:10 (2012)
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