G2404
Anti-Golgi FTCD antibody, clone 58k-9, Mouse monoclonal
ascites fluid
Sinônimo(s):
Anti-LCHC1
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About This Item
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
peso molecular
antigen 58 kDa
contém
15 mM sodium azide
reatividade de espécies
human, bovine, mouse, pig, canine, kangaroo rat, hamster, monkey, rat
técnica(s)
electron microscopy: suitable
indirect immunofluorescence: 1:50 using cultured CHO cells
western blot: 1:5,000 using whole rat liver extract
Isotipo
IgG1
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... FTCD(10841)
mouse ... Ftcd(14317)
rat ... Ftcd(89833)
Descrição geral
FTCD is a homooctamer with its subunits arranged in a planar ring. It is predominantly expressed in the liver. Apart from being associated with the Golgi apparatus, it is detectable in the supernatant cytosolic fraction, and may also be localized to cytoplasmic vesicles.
Monoclonal Anti-Golgi 58K Protein/formiminotransferase cyclodeaminase (FTCD) (mouse IgG1 isotype) is derived from the 58K-9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with FTCD/Golgi 58K protein purified from rat liver.
Especificidade
The antibody recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. It is also useful for studies on the effect of microtubule-perturbing agents on the Golgi apparatus.
Imunogênio
Golgi 58K protein from rat liver.
Aplicação
Anti-Golgi FTCD antibody, clone 58k-9, Mouse monoclonal has been used in western blot analysis and dual immunofluorescence staining.
Anti-Golgi FTCD antibody, clone 58k-9, Mouse monoclonal is suitable for electron microscopy, indirect immunofluorescence at a working dilution of 1:50 using cultured CHO cells and indirect immunoblotting at 1:5000 working dilution using whole rat liver extract. It is also useful for the localization of Golgi 58K protein using immunoblotting, dot blot, electron microscopy, and immunocytochemistry.
The antibody was used:
The antibody was used:
- for the analysis of distribution of the 58K9 protein exclusively localized in the Golgi
- as a primary antibody in the immunofluorescence analysis in studies related to functioning of nuclear envelope protein TMEM209 in lung carcinoma cells, tracking TG2 (Transglutaminase Type 2) transport in renal tubular epithelial cells, binding of TRADD (TNFR-associated death domain protein) to TNF-R1 at the plasma membrane and localization of Wilson disease protein in the Golgi apparatus
- in immunoprecipitation studies
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Ações bioquímicas/fisiológicas
FTCD/58K protein of the Golgi apparatus used in conjunction with other antibodies to Golgi proteins (e.g., the Golgi β-COP protein) may be used for studies on the role and relationships of this protein in the cell. It is also useful for localization studies following subcellular fractionation procedures.
Golgi 58K protein is associated with the Golgi apparatus peripherally and has been identified as a version of FTCD (Formiminotransferase Cyclodeaminase). It is a bifunctional metabolic enzyme involved in conversion of histidine to glutamic acid. It acts as a channel for the transport of one-carbon units from formiminoglutamate to the folate pool. It also interacts with vimentin subunits and with polymerized vimentin filaments. Autosomal recessive disorder glutamate formiminotransferase deficiency is caused by defects in FTCD.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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Frantisek Baluska et al.
Plant physiology, 130(1), 422-431 (2002-09-13)
Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response to BFA treatment is a unique feature of some, but
Human Cathepsin W, a Cysteine Protease Predominantly Expressed in NK Cells, Is Mainly Localized in the Endoplasmic Reticulum
Wex T, et al.
Journal of Immunology, 167(4), 2172-2178 (2001)
S J Jones et al.
Journal of immunology (Baltimore, Md. : 1950), 162(2), 1042-1048 (1999-01-23)
The subcellular localization of TNF-R1 to the Golgi apparatus, initially observed in endothelial cells, has been confirmed using transfection of bovine aortic endothelial cells with a human TNF-R1 expression plasmid. The subcellular interactions of TNF-R1 and the TRADD (TNFR-associated death
Jonathan H Clarke et al.
The Journal of comparative neurology, 517(3), 296-312 (2009-09-17)
The role of cellular phosphatidylinositol 5-phosphate (PtdIns5P), as a signalling molecule or as a substrate for the production of small, compartmentalized pools of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)], may be dependent on cell type and subcellular localization. PtdIns5P levels are primarily regulated
Ya-Sheng Gao et al.
European journal of cell biology, 81(7), 391-401 (2002-08-06)
Whether the highly dynamic structure of the vimentin intermediate filament (IF) cytoskeleton responds to cues from cellular organelles, and what proteins might participate in such events is largely unknown. We have shown previously that the Golgi protein formiminotransferase cyclodeaminase (FTCD)
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