U6382

Anti-UCP-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Ucp1 Antibody, Ucp1 Antibody - Anti-UCP-1 antibody produced in rabbit, Anti-Uncoupling Protein 1
• Número MDL: MFCD01092514
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 300
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~32 kDa
• reatividade de espécies: mouse, human, rat
• embalagem: antibody small pack of 25 μL
• técnica(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of mouse brown adipose tissue.; western blot: 1:1,000 using mitochondrial extract of rat brown adipose tissue
• nº de adesão UniProt: P25874
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... UCP1(7350) ; mouse ... Ucp1(22227) ; rat ... Ucp1(24860)

Descrição

Descrição geral

Uncoupling protein (UCP1) is a 32 kDa, mitochondrial inner membrane protein. It is active as a proton channel forming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids.

Especificidade

Anti-UCP-1 specifically recognizes human, mouse and rat UCP-1. Staining of the UCP-1 band is specifically inhibited with the immunizing peptide. The epitopes recognized are compatible with routine formalin-fixation and paraffin-embedding.

Imunogênio

synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in rat and mouse UCP1.

Aplicação

Anti-UCP-1 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Ações bioquímicas/fisiológicas

Uncoupling proteins (UCPs) are transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They can return protons pumped by the respiratory chain to the mitochondrial matrix. UCP-1 is exclusively expressed in brown adipose tissue (BAT) in rodents and in neonates where it is regulated by norepinephrine and thyroid hormones. UCP-1 has been shown to depend on CoQ (ubiquinone) as an obligatory co-factor.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3