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B8931

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

Sinônimo(s):

Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside

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About This Item

Fórmula empírica (Notação de Hill):
C14H15BrClNO6
Número CAS:
93863-88-8
Peso molecular:
408.63
Número MDL:
MFCD02683897
Código UNSPSC:
12352200
ID de substância PubChem:
329773145
NACRES:
NA.85

grau

for molecular biology

Nível de qualidade

200

esterilidade

non-sterile

linha de produto

BioReagent

Ensaio

≥98.0% (HPLC)

Formulário

powder

solubilidade

methanol: soluble

adequação

suitable for substrate for β-galactosidase

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1

chave InChI

CHRVKCMQIZYLNM-HTOAHKCRSA-N

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Descrição geral

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Aplicação

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.

Princípio

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
BCBK8608V
BCBJ5700V
1403239V
1403239
102K2506

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Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

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