C4478
S-Gal®/LB Agar Blend
reagent for selection of recombinant bacterial clones
Sinônimo(s):
Agar Blend
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About This Item
Código UNSPSC:
41106200
NACRES:
NA.85
Produtos recomendados
grau
for molecular biology
esterilidade
non-sterile
forma
powder
técnica(s)
microbiological culture: suitable
adequação
suitable for β-galactosidase test
nonselective for Escherichia coli
nonselective for coliforms
aplicação(ões)
food and beverages
microbiology
temperatura de armazenamento
room temp
Categorias relacionadas
Descrição geral
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Aplicação
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
Características e benefícios
- More intense color contrast than X-gal
- Water-soluble and autoclavable for easiest use
- Convenient, pre-mixed media
Componentes
Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
Princípio
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Nota de preparo
Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.
Outras notas
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Informações legais
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
produto relacionado
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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