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B1022

Sigma-Aldrich

Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt

≥90% (TLC), powder

Sinônimo(s):

Sulfosuccinimidyl 6-(biotinamido)hexanoate

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About This Item

Fórmula empírica (Notação de Hill):
C20H29N4NaO9S2
Número CAS:
Peso molecular:
556.59
Número MDL:
Código UNSPSC:
12352203
ID de substância PubChem:
NACRES:
NA.46

Nível de qualidade

Ensaio

≥90% (TLC)

Formulário

powder

solubilidade

H2O: 10 mg/mL
DMF: 50 mg/mL

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

[Na].OS(=O)(=O)C1CC(=O)N(OC(=O)CCCCCNC(=O)CCCCC2SCC3NC(=O)NC23)C1=O

InChI

1S/C20H30N4O9S2.Na.H/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32;;/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32);;

chave InChI

KDRVGWAQYRWAJF-UHFFFAOYSA-N

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Descrição geral

Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt is a water soluble biotinylation reagent. It can be used in immunoprecipitation of signaling proteins. It can also be used in analysis of hyaluronan synthesis. Further, the product can also be used in ELISA. This biotinylation reagent has shown specificity for human dermal fibroblasts.

Aplicação

Water soluble biotinylation reagent incorporating an aminocaproyl spacer. This can reduce the steric hindrance in binding avidin to some biotinylated compounds. Has also been used as a cell surface labeling reagent. Typically coupled to primary amine in the pH range 6.5-8.5. May be used for the biotinylation of cell surface proteins.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Repr. 1B

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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H Watarai et al.
Electrophoresis, 21(2), 460-464 (2000-02-16)
The expression of plasma membrane proteins in human monocyte-derived U937 cells was examined by cell disruption and isolation of microsomal fractions. Two alternative procedures for cell disruption, Dounce homogenization and nitrogen cavitation, were compared. Cell homogenization and sequential centrifugation resulted
A V Vener et al.
Analytical biochemistry, 191(1), 65-69 (1990-11-15)
A colorimetric procedure to detect biotin bound to microtiter plates with a sensitivity down to 10(-16) mol was developed using biotinylated inorganic pyrophosphatase of Escherichia coli. Reaction of pyrophosphatase with 1 mM N-biotinyl-6-aminocaproic acid N-hydroxy-sulfonosuccinimide ester yielded a stable 87%
Improved nonradioactive cell surface labeling technique for immunoprecipitation.
L M Lantz et al.
BioTechniques, 18(1), 56-56 (1995-01-01)
Mohammed M Nooh et al.
American journal of physiology. Renal physiology, 317(2), F375-F387 (2019-05-30)
Arginine-vasopressin (AVP)-mediated translocation of aquaporin-2 (AQP2) protein-forming water channels from storage vesicles to the membrane of renal collecting ducts is critical for the renal conservation of water. The type-1 PDZ-binding motif (PBM) in AQP2, "GTKA," is a critical barcode for
Lingli Li et al.
The Journal of biological chemistry, 281(36), 26512-26519 (2006-07-01)
The extracellular matrix molecule hyaluronan was found to suppress platelet-derived growth factor (PDGF) beta-receptor activation and PDGF-BB-induced migration of primary human dermal fibroblasts. The suppressive effect of hyaluronan was neutralized by a monoclonal antibody that specifically inhibits hyaluronan binding to

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