APPA014

HIV Protease Activity Detection Kit

• Sinônimo(s): Cut-N-Glow HIV Protease Detection Kit
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• forma: solution
• Nível de qualidade: 200
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Aplicação

This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.

Adequação

This kit contains sufficient reagents for one 96 well plate (96 tests)

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable