SRP2152
HIV Protease, His tagged,recombinant from HIV-1
recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Sinônimo(s):
HIV_retropepsin_like, Retropepsins, cd05482, pepsin-like aspartate proteases
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352204
NACRES:
NA.26
Produtos recomendados
fonte biológica
human immunodeficiency virus 1
recombinante
expressed in E. coli
Ensaio
≥85% (SDS-PAGE)
forma
frozen liquid
peso molecular
~11.9 kDa
embalagem
pkg of 10 μg
condição de armazenamento
avoid repeated freeze/thaw cycles
concentração
200 μg/mL
cor
colorless to clear
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Aplicação
HIV Protease has been used in in vitro detection of HIV protease activity using FRET-HIV Sensor, a Förster resonance energy transfer-based HIV protease-sensitive sensor.
Ações bioquímicas/fisiológicas
The HIV-1 core consists of a viral genome housed within a conical viral capsid that is generated during virion maturation. Human immunodeficiency virus type 1 (HIV-1) matures after the viral protease processes the Gag and Pol polyproteins at 10 substrate locations. The protease of HIV-1 is an aspartic protease and is functional only as a dimer; dimerization results in the formation of a binding cleft in which each of the two catalytic aspartic acids is contributed by one of the monomers. Because the protease is active only as a dimer, two of the GagPol precursors must themselves dimerize during virus assembly so that their protease domains can dimerize, become active, and process the precursors. The order and kinetics of cleavage as well as the extent of precursor processing appear to be critical steps in the generation of fully infectious, appropriately assembled viral particles. Inhibition of HIV-1 protease represents an important avenue for antiviral therapy. Currently available combination chemotherapy with reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs) for human immunodeficiency virus type 1 (HIV-1) infection and AIDS have been shown to suppress the replication of HIV-1 and extend the life expectancy of HIV-1-infected individuals.
Sequência
PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF
forma física
Clear and colorless frozen liquid solution
Formulated in 20mM Mes buffer, pH6, 500mM KCl, 20% glycerol.
Nota de preparo
Please keep in −20 °C for long term storage. It lost 70% activity in 4°C for one week. Freeze thaw cycle resistance.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
C Peng et al.
Journal of virology, 63(6), 2550-2556 (1989-06-01)
It is generally believed that the gag gene product of human immunodeficiency virus type 1 (HIV-1) is processed into several core proteins by a virus-specific protease. We used deletion mutation analysis to study the role of HIV-specific protease in the
K C Chou et al.
Proteins, 24(1), 51-72 (1996-01-01)
Based on the sequence-coupled (Markov chain) model and vector-projection principle, a discriminant function method is proposed to predict sites in protein substrates that should be susceptible to cleavage by the HIV-1 protease. The discriminant function is defined by delta =
Noninvasive high-throughput single-cell analysis of HIV protease activity using ratiometric flow cytometry.
Gaber R
Sensors (Basel, Switzerland), 13, 16330-16346 (2013)
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica