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APF

Sigma-Aldrich

Alkaline Phosphatase Detection Kit, Fluorescence

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About This Item

Número CE:
Código UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.53

grau

for molecular biology

Nível de qualidade

uso

 kit sufficient for 300 reactions (200 μl reactions)

técnica(s)

microbe id | specific enzyme detection: suitable

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... ALPL(249)

Descrição geral

The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.

Aplicação

Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity.
Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts.

Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Ações bioquímicas/fisiológicas

Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways.

Características e benefícios

  • Up to 100x more sensitive than colorimetric assays
  • SEAP can be measured without sample destrutcion
  • Rapid and reproducible results
  • Safe and easy-to-use

Componentes

The Alkaline Phosphatase Kit contains:
50mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168)

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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