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MAK055

Sigma-Aldrich

AST Activity Assay Kit

sufficient for 100 colorimetric tests

Sinônimo(s):

Aspartate Aminotransferase Activity Assay Kit, Glutamate-Oxaloacetate Transaminase Activity Assay Kit, SGOT Activity Assay Kit, Serum Glutamate-Oxaloacetate Transaminase Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detecção

colorimetric

doença(s) relevante(s)

gastrointestinal diseases; cardiovascular diseases

temperatura de armazenamento

−20°C

Informações sobre genes

human ... GOT1(2805)
mouse ... GOT1(14718)
rat ... GOT1(24401)

Categorias relacionadas

Descrição geral

The new AST Assay Kit, MAK467, is now available! Aspartate Aminotransferase (AST), formerly called serum glutamate-oxaloacetate transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Similar to Alanine Aminotransferase (ALT), AST levels in blood are commonly used as a marker for liver function. However, AST has a broader tissue distribution than ALT and perturbations in AST levels can occur in response to diseases or injuries in multiple tissues including skeletal and heart.

Aplicação

AST Activity Assay Kit has been used to determine the activity of aspartate aminotransferase in samples.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the measurement of aspartate aminotransferase (AST) activity in biological samples including tissue, cells and serum

Princípio

The Aspartate Aminotransferase Activity Assay kit provides a simple and direct procedure for measuring AST activity in a variety of samples. In this kit, the transfer of an amino group from aspartate to α-ketoglutarate results in the generation of glutamate, resulting in the production of a colorimetric (450 nm) product proportional to the AST enzymatic activity present. One unit of AST is the amount of enzyme that will generate 1.0 μmole of glutamate per minute at pH 8.0 at 37 °C.

substituído por

Nº do produto
Descrição
Preços

Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palavra indicadora

Danger

Frases de perigo

H314,H334

Classificações de perigo

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

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Certificados de análise (COA)

Lot/Batch Number
7K13K07530
7F21K07530
7B17K07530
6K29K07530
6J04K07530

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Sigma-Aldrich

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