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Documentos Principais

MAK116

Sigma-Aldrich

Creatine Kinase Activity Assay Kit

sufficient for 100 tests (Ultraviolet)

Sinônimo(s):

CK Activity Assay Kit, Creatine Phosphokinase Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 tests (Ultraviolet)

método de detecção

colorimetric

doença(s) relevante(s)

cardiovascular diseases; genitourinary disorders; aging/geriatric diseases; orthopedic diseases

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... CKB(1152) , CKM(1158)
mouse ... CKB(12709) , CKM(12715)
rat ... CKB(24264) , CKM(24265)

Categorias relacionadas

Descrição geral

Creatine Kinase (CK), also known as phosphocreatine kinase, is an enzyme that catalyzes the transfer of one phosphate group from ATP to creatine generating phosphocreatine, an important energy reservoir in muscle and brain tissue. CK is a dimeric protein made up of B (brain) and M (muscle) subunits. Three isoenzymes, CK-MM, CK-MB, and CK-BB, have been observed. CK levels are elevated in various pathological conditions including myocardial infarction, rhabdomyolysis, muscular dystrophy, and renal failure.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the detection of Creatine Kinase activity in a variety of samples such as serum and plasma. This kit can be used to study the effects of various compounds on Creatine Kinase activity.

Princípio

Creatine Kinase activity is determined by a coupled enzyme reaction resulting in the production of NADPH, measured at 340 nm, proportionate to the CK activity present in the sample. In this reaction, phosphocreatine and ADP are converted to creatine and ATP. The generated ATP is used by hexokinase to phosphorylate glucose resulting in glucose-6-phosphate, which is oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase to produce NADPH and 6-phospho-D-gluconate. One unit of CK is the amount of enzyme that will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per minute at pH 6.0. This kit has a linear range of 30–1,800 units/L CK activity.

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
116CB08A17
116CB07A22
116CB04A08
116CA12A10
116CA09A03

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