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Key Documents

MAK095

Sigma-Aldrich

Aspartate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

método de detecção

colorimetric
fluorometric

doença(s) relevante(s)

endocrinological disorders, diabetes; neurological disorders

temperatura de armazenamento

−20°C

Descrição geral

Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.

Aplicação

Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells and Lactococcus lactis strain cultured in lysogeny broth (LB) medium.

Adequação

The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Princípio

Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

substituído por

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

188.6 °F - closed cup

Ponto de fulgor (°C)

87 °C - closed cup


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Essentials of medical biochemistry: with clinical cases.
Ha C E and Bhagavan N V
ESSENTIALS OF MEDICINAL CHEMISTRY, 2011 null
Role of the Malate-Aspartate Shuttle on the Metabolic Response to Myocardial Ischemia
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