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Key Documents

QPBCA

Sigma-Aldrich

QuantiPro BCA Assay Kit

for 0.5-30 μg/ml protein

Sinônimo(s):

BCA Assay, Protein determination by BCA, bicinchoninic acid reagent

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

Nível de qualidade

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Aplicação

In addition to protein determination and quantification in solution, the QuantiPro BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Características e benefícios

  • Accurate across a broad range of protein concentrations
  • High sensitivity; linear response form 0.5 to 30 μg/ml of protein
  • Stable color complex
  • Reduced susceptibility to detergents

Princípio

Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.

Armazenamento e estabilidade

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Informações legais

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Somente componentes do kit

Nº do produto
Descrição

  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS

Aplicação

Nº do produto
Descrição
Preços

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Pictogramas

CorrosionEnvironment

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Conteúdo relacionado

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