Western Blot Analysis of Immunoprecipitation (IP-Western)
IP-Western analysis remains a popular technique for identifying protein-protein interactions and identifying unknown proteins in a multi-protein complex. The steps include cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.
Click on the Western Blot Analysis of Immunoprecipitation (IP-Western) topics to read about the possible causes and remedies:
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Materials
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