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Documentos Principais

A8917

Sigma-Aldrich

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinônimo(s):

Rabbit Anti-Bovine IgG (whole molecule)−HRP

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

rabbit

Nível de qualidade

conjugado

peroxidase conjugate

forma do anticorpo

IgG fraction of antiserum

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

forma

buffered aqueous solution

reatividade de espécies

bovine

técnica(s)

direct ELISA: 1:20,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

Immunoglobulin G (IgG) contributes to 10−20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Imunogênio

purified bovine IgG

Aplicação

Anti-Bovine IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in:
  • Dot- enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistology

Rabbit Anti-Bovine IgG (whole molecule)-Peroxidase antibody has been used for western blot and ELISA. The antibody can also be used for dot blot (1:80,000) and immunohistochemistry (1:1,000).

Ações bioquímicas/fisiológicas

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Nota de preparo

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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