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Documentos Principais

A6684

Sigma-Aldrich

Monoclonal Anti-Albumin antibody produced in mouse

clone HSA-11, ascites fluid

Sinônimo(s):

Anti-AFP

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

HSA-11, monoclonal

contém

15 mM sodium azide

reatividade de espécies

gibbon, baboon, human, rhesus monkey

não deve reagir com

turkey, feline, hamster, pigeon, rat, marmoset, pig, bovine, canine, donkey, guinea pig, rabbit, chicken, sheep, goat

técnica(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: 1:10,000
western blot: 1:5,000

Isotipo

IgG2a

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ALB(213)

Descrição geral

Monoclonal Anti-Albumin (mouse IgG2a isotype) is derived from the HSA-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Albumin, the major protein produced by the liver, represents more than half the total protein in human serum. Many other body fluids also contain albumin.
Serum albumin is the major protein component of blood and is produced in the liver. It regulates colloid osmotic blood pressure, transportation of a variety of substances, blood pH and provides an endogenous source of amino acids. Monoclonal anti-albumin antibody (diluted 1:100) can be used in double staining to localize hepatocytes differentiated from human umbilical mesenchymal stem cells (HUMSCs) . Offering specificity for human serum albumin, this product is ideal for identification of human blood stains or other body fluids (e.g., seminal fluid, milk, urine, saliva, vaginal secretions). Mouse monoclonal anti-albumin antibody reacts specifically with denatured and reduced human serum albumin and cross reacts with rhesus, gibbon and baboon serum albumins. The product has shown no cross reactivity with bovine, cat, chicken, dog, donkey, goat, guinea pig, hamster, marmoset, pig, pigeon, rabbit, rat, sheep, or turkey serum albumin or with chicken and turkey egg albumin.

Especificidade

The antibody is specific for human albumin in serum and plasma and recognizes an epitope resistant to reduction or denaturation.

Imunogênio

Human serum albumin

Aplicação

Monoclonal Anti-Albumin antibody produced in mouse has been used in:
  • immunofluorescence
  • staining
  • double staining
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • immunohistochemistry

Ações bioquímicas/fisiológicas

Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes .
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Oxidative damage of albumin is associated with advanced liver disease.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Clinical use of albumin
Caraceni P, et al.
Blood Transfusion = Trasfusione del Sangue, 11(Suppl 4), s18-s18 (2013)
Katy M Olsavsky et al.
Toxicology and applied pharmacology, 222(1), 42-56 (2007-05-22)
Frequently, primary hepatocytes are used as an in vitro model for the liver in vivo. However, the culture conditions reported vary considerably, with associated variability in performance. In this study, we characterized the differentiation character of primary human hepatocytes cultured
Efficient generation of functional hepatocyte-like cells from human fetal hepatic progenitor cells in vitro
Zhang W, et al.
Journal of Cellular Physiology, 227(5), 2051-2058 (2012)
Yoon Ok Jang et al.
BMC gastroenterology, 14, 198-198 (2014-11-27)
Cirrhosis is a long-term consequence of chronic hepatic injury with fibrosis. No effective therapy is currently available for decompensated cirrhosis except liver transplantation. Hence, we investigated the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on hepatic fibrosis in a
Obinna C Ubah et al.
Frontiers in immunology, 8, 1780-1780 (2018-01-10)
The management of chronic inflammatory diseases, such as inflammatory bowel disease, psoriasis, and rheumatoid arthritis has significantly improved over the last decade with the clinical availability of anti-TNF-α biologics. Despite this undoubted treatment success, a combination of acquired resistance together

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