A4325
Monoclonal Anti-α-Adaptin antibody produced in mouse
clone 100/2, ascites fluid
Sinônimo(s):
Anti-AP-2
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About This Item
Produtos recomendados
fonte biológica
mouse
conjugado
unconjugated
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
100/2, monoclonal
contém
15 mM sodium azide
reatividade de espécies
bovine, human, rat
técnica(s)
western blot: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex
Isotipo
IgG2a
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... AP2A1(160)
rat ... Ap2a1(308578)
Descrição geral
Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.
Monoclonal Anti-a-Adaptin (AP-2) (mouse IgG2a isotype) is derived from the 100/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptor related protein complex 2 subunit alpha 1 (AP2A1) codes for αA adaptin of the AP-2 complex. It is usually seen in clathrin coated vesicles.
The antibody reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact α-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of α-fragments obtained by trypsin cleavage of the α-subunit molecules.
Imunogênio
AP-2 adaptor polypeptides from bovine brain.
Aplicação
Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.
Monoclonal Anti-α-Adaptin antibody produced in mouse has been used in immunoblotting.
Mouse monoclonal anti-α-adaptin antibody has been used:
- as a positive control in immunoblotting assays
- as a primary antibody to detect AP2 levels in tetracycline-repressible stable cell lines
Ações bioquímicas/fisiológicas
Adaptor related protein complex 2 subunit α 1 (AP2α1) is very essential for epidermal growth factor receptor (EGFR) nuclear translocation, induced by ultraviolet (UV).
forma física
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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R A Warren et al.
The Journal of biological chemistry, 273(27), 17056-17063 (1998-06-27)
Endocytosis of surface proteins through clathrin-coated pits requires an internalization signal in the cytoplasmic domain. Two types of internalization signal have been described: one requiring a tyrosine as the critical residue (tyrosine-based motif), and the other consisting of either two
Molecular cloning of novel transcripts of the adaptor-related protein complex 2 alpha 1 subunit (AP2A1) gene, using Next-Generation Sequencing
Adamopoulos PG, et al.
Gene, 678, 55-64 (2018)
Rajesh Abraham Jacob et al.
Virology, 509, 1-10 (2017-06-04)
Acquired Immune Deficiency Syndrome is characterized by a decline in CD4
H C van Leeuwen et al.
Journal of cell science, 114(Pt 11), 2115-2123 (2001-08-09)
p32/gC1qR is a small acidic protein that has been reported to have a broad range of distinct functions and to associate with a wide array of cellular, viral and bacterial proteins. It has been found in each of the main
M S Robinson
Trends in cell biology, 2(10), 293-297 (1992-10-01)
Clathrin has long been known to provide the structural basis for vesicle budding from the plasma membrane during endocytosis, but how is clathrin targeted specifically to some cellular membranes and not others? The answer seems to lie in the adaptors--protein
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