OP13
Anti-N-Myc Mouse mAb (NCM II 100)
liquid, clone NCM II 100, Calbiochem®
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
NCM II 100, monoclonal
Formulário
liquid
não contém
preservative
reatividade de espécies
human, mouse
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG1
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... MYCN(4613)
Descrição geral
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.
Recognizes the N-myc protein and its cleavage product in IMR5 cells.
This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.
Imunogênio
Human
recombinant, human, N-myc fusion protein, expressed in E. coli
Aplicação
Frozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Embalagem
Please refer to vial label for lot-specific concentration.
Advertência
Toxicity: Standard Handling (A)
forma física
In 50 mM sodium phosphate buffer, 50% glycerol.
Reconstituição
Following initial thaw, aliquot and freeze (-20°C).
Nota de análise
Positive Control
IMR5 cells
IMR5 cells
Outras notas
Brondyk, W.H. 1991. Oncogene6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Viktoryia Sidarovich et al.
Molecular biotechnology, 56(7), 631-643 (2014-02-12)
Neuroblastoma is the most common extracranial solid tumor of infancy. Amplification of MYCN oncogene is found in approximately 20 % of all neuroblastoma patients and correlates with advanced disease stages, rapid tumor progression, and poor prognosis, making this gene an
H P Wang et al.
Journal of virology, 72(3), 2192-2198 (1998-03-14)
Cell line WH44KA is a highly malignant woodchuck hepatoma cell line. WH44KA cells contain a single woodchuck hepatitis virus (WHV) DNA integration in the 3' untranslated region of exon 3 of the woodchuck N-myc1 gene. The highly rearranged WHV DNA
Erika A Newman et al.
Cell death & disease, 8(12), 3208-3208 (2017-12-15)
Neuroblastoma is a cancer of neural crest stem cell (NCSC) lineage. Signaling pathways that regulate NCSC differentiation have been implicated in neuroblastoma tumorigenesis. This is exemplified by MYCN oncogene targets that balance proliferation, differentiation, and cell death similarly in normal
James Chappell et al.
Genes & development, 27(7), 725-733 (2013-04-18)
Suppression of extracellular signal-regulated kinase (ERK) signaling is an absolute requirement for the maintenance of murine pluripotent stem cells (mPSCs) and requires the MYC-binding partner MAX. In this study, we define a mechanism for this by showing that MYC/MAX complexes
Giovanni Perini et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(34), 12117-12122 (2005-08-12)
N-Myc is a transcription factor that forms heterodimers with the protein Max and binds gene promoters by recognizing a DNA sequence, CACGTG, called E-box. The identification of N-myc target genes is an important step for understanding N-Myc biological functions in
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica