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A3641

Sigma-Aldrich

Apoferritin from equine spleen

0.2 μm filtered

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About This Item

Número CAS:
9013-31-4
Número MDL:
MFCD00081365
Código UNSPSC:
12352202
NACRES:
NA.32

Nível de qualidade

200

esterilidade

0.2 μm filtered

forma

liquid

peso molecular

major subunit ML 19,889
minor subunit MH 22,200
native ~481.2 kDa (24 subunits, approx. 20 kDa each)

cor

clear to slightly hazy, solution

temperatura de armazenamento

2-8°C

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Aplicação

Apoferritin from equine spleen has been used:
  • to determine its partition coefficients in phase systems
  • in NaCl for iron loading of cultured cells
  • in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology

Ações bioquímicas/fisiológicas

This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant. When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis.

forma física

Solution in 0.135 M sodium chloride.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves

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Circularly polarized luminescent CdS quantum dots prepared in a protein nanocage.
Masanobu Naito et al.
Angewandte Chemie (International ed. in English), 49(39), 7006-7009 (2010-08-27)
Ferritin-iron increases killing of Chinese hamster ovary cells by X-irradiation
Nelson JM and Stevens RG
Cell proliferation, 25(6), 579-585 (1992)
Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy
Evans JE, et al.
Micron (Oxford, England : 1993), 43(11), 1085-1090 (2012)
M J MacDonald et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 8(10), 777-781 (1994-07-01)
In screening a rat pancreatic islet cDNA library by differential hybridization for transcripts increased by glucose, the H chain of ferritin, an iron storage protein, was identified. An ELISA for rat ferritin showed that the insulin cell contains a surprisingly
G Balla et al.
The Journal of biological chemistry, 267(25), 18148-18153 (1992-09-05)
Phagocyte-mediated oxidant damage to vascular endothelium is likely involved in various vasculopathies including atherosclerosis and pulmonary leak syndromes such as adult respiratory distress syndrome. We have shown that heme, a hydrophobic iron chelate, is rapidly incorporated into endothelial cells where
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