A0856

Anti-ATG5 (N-terminal) antibody produced in rabbit

affinity isolated antibody, PBS solution

• Sinônimo(s): ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit, Atg5 Antibody, Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like
• Número MDL: MFCD09753847
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: PBS solution
• peso molecular: antigen ~56 kDa (Atg5-Atg12 complex)
• reatividade de espécies: mouse, human, rat
• embalagem: antibody small pack of 25 μL
• técnica(s): immunofluorescence: suitable; western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells
• nº de adesão UniProt: Q9H1Y0
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ATG5(9474) ; mouse ... Atg5(11793) ; rat ... Atg5(365601)

Descrição

Especificidade

Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

Imunogênio

synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Aplicação

Anti-ATG5 (N-terminal) antibody has been used:

• in immunoblotting
• in immunofluorescence
• in western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

Ações bioquímicas/fisiológicas

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)