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A0412

Sigma-Aldrich

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat

affinity isolated antibody

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

goat

Nível de qualidade

conjugado

peroxidase conjugate

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

reatividade de espécies

mouse

técnica(s)

direct ELISA: 1:10,000 using IgG, IgA, IgM

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria, and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells,,. Goat anti-mouse polyvalent immunoglobulins (G,A,M)-peroxidase antibody binds to mouse IgG, IgA and IgM.

Aplicação

The detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary. Relative binding affinities for specific Mabs were determined by an inhibition ELISA using peroxidase-conjugated goat anti-mouse polyvalent antibody diluted 1:10,000 in PBST and incubated for 1 hour at 21-23°C.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Nota de preparo

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Frases de perigo

Classificações de perigo

Aquatic Chronic 3 - Skin Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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