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Key Documents

86R

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast red violet LB

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About This Item

Código UNSPSC:
12352106
eCl@ss:
42010105
NACRES:
NA.47

Nível de qualidade

prazo de validade

Expiry date on the label.

IVD

for in vitro diagnostic use

aplicação(ões)

hematology
histology

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

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Aplicação

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast red violet LB. The resulting insoluble diffuse, red dye deposit indicates sites of alkaline phosphatase activity.

Somente componentes do kit

Nº do produto
Descrição

  • Citrate Solution (915) 50 mL

  • FRV-Alkaline Solution (862) 10 mL

  • Hematoxylin Solution, Gill No. 3 (kit only) 50 mL

  • Naphthol AS-BI Alkaline Solution (861) 10 mL

  • Sodium Nitrite Solution (914) 10 mL

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - STOT RE 2 Oral

Órgãos-alvo

Kidney

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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The development of a new family of implantable bioinspired materials is a focal point of bone tissue engineering. Implant surfaces that better mimic the natural bone extracellular matrix, a naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages
R Raz et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(6), 2846-2851 (1999-03-17)
Propagation of mouse embryonic stem (ES) cells in vitro requires exogenous leukemia inhibitory factor (LIF) or related cytokines. Potential downstream effectors of the LIF signal in ES cells include kinases of the Src, Jak, and mitogen-activated protein families and the
Edgardo Rodríguez-Carballo et al.
PloS one, 9(7), e102032-e102032 (2014-07-10)
p38 MAPK activity plays an important role in several steps of the osteoblast lineage progression through activation of osteoblast-specific transcription factors and it is also essential for the acquisition of the osteoblast phenotype in early development. Although reports indicate p38
Salvatore Patanè et al.
Cancer research, 66(9), 4750-4757 (2006-05-03)
The MET oncogene was causally involved in the pathogenesis of a rare tumor, i.e., the papillary renal cell carcinoma, in which activating mutations, either germline or somatic, were identified. MET activating mutations are rarely found in other human tumors, whereas
Terence P Gade et al.
PloS one, 6(7), e22608-e22608 (2011-07-30)
The purpose of this study was to develop a paradigm for quantitative molecular imaging of bone cell activity. We hypothesized the feasibility of non-invasive imaging of the osteoblast enzyme alkaline phosphatase (ALP) using a small imaging molecule in combination with

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