83702

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

• Sinônimo(s): 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
• Fórmula linear: C₂₁H₁₇ClN₂O₃
• Número CAS: 62669-70-9
• Peso molecular: 380.82
• Beilstein: 6030951
• Número CE: 263-687-8
• Número MDL: MFCD00012664
• Código UNSPSC: 12352116
• ID de substância PubChem: 57652966
• NACRES: NA.32

Propriedades

• grau: for fluorescence
• linha de produto: BioReagent
• Ensaio: ≥85% (HPLC)
• Formulário: powder
• Impurezas: ≤10% water
• solubilidade: ethanol: 20 mg/mL, clear, red
• adequação: in accordance for fluorescence; suitable for fluorescence
• cadeia de caracteres SMILES: Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
• InChI: 1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H
• chave InChI: MYFATKRONKHHQL-UHFFFAOYSA-N

Descrição

Descrição geral

Rhodamine 123 is a fluorescent dye.

Aplicação

Rhodamine 123 has been used:

• as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
• to label the cells for estimating the P-gp expression by flow cytometry
• in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
• the detection of mitochondrial membrane potential (ΔΨm)

Ações bioquímicas/fisiológicas

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

Outras notas

Supravital mitochondrial-specific dye

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)