D1054

Dihydrorhodamine 123

≥95% purity, powder

• Sinônimo(s): DHR 123; Methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate; Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester
• Fórmula empírica (Notação de Hill): C₂₁H₁₈N₂O₃
• Número CAS: 109244-58-8
• Peso molecular: 346.38
• Beilstein: 5989965
• Número MDL: MFCD00285712
• Código UNSPSC: 12171500
• ID de substância PubChem: 24893337
• NACRES: NA.47

Propriedades

• product name: Dihydrorhodamine 123, ≥95%
• Nível de qualidade: 200
• Ensaio: ≥95%
• forma: powder
• cor: pink
• solubilidade: DMSO: >5 mg/mL
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: COC(=O)c1ccccc1C2c3ccc(N)cc3Oc4cc(N)ccc24
• InChI: 1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
• chave InChI: FNEZBBILNYNQGC-UHFFFAOYSA-N

Descrição

Descrição geral

Dihydrorhodamine 123 (DHR123) is an uncharged non-fluorescent dye and a derivative of rhodamine 123 (R123). Dihydrorhodamine 123 (DHR123) is oxidized to fluorescent R123 within cells in the presence of reactive oxygen species and it localizes in mitochondria. It is an analog of 2,7-Dichlorodihydrofluorescein (DCDHF), lipophilic and diffuses through cell membrane.

Aplicação

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

Ações bioquímicas/fisiológicas

Dihydrorhodamine 123 detects superoxide ions at the single-cell level and is used for probing neutrophils superoxide generation in a respiratory burst event. It is also used for measuring oxidative stress in macrophages and human spermatozoa.

Dihydrorhodamine 123 is used for the detection of ROS (reactive oxygen species). Presence of ROS oxidizes dihydrorhodamine 123 to the fluorescent derivative rhodamine 123. The reaction happens in the presence of a peroxidase or a similar catalyst.

Substratos

Peroxidase substrate

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)