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H4290

Sigma-Aldrich

Hydroxyphenyl fluorescein solution

5 mM in DMF

Sinônimo(s):

Hydroxyphenyl fluorescein, 2-[6-(4′-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid, HPF

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About This Item

Fórmula empírica (Notação de Hill):
C26H16O6
Peso molecular:
424.40
Número CE:
200-679-5
Código UNSPSC:
12352108
NACRES:
NA.32

concentração

5 mM in DMF

fluorescência

λex 490 nm; λem 515 nm

temperatura de armazenamento

2-8°C

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Descrição geral

Hydroxyphenyl fluorescein solution, also known as HPF is a fluorescent probe, which upon oxidation by the hydroxyl radical (HO) returns a brilliant green fluorescence (HPF-ox). Increased HPF signals in antibiotic-exposed bacterial cells are explained by fluorescence associated with increased cell size, not by ROS levels. In cells incubated with the HPF probe and exposed to ampicillin and norfloxacin with HPF fluorescence correlated linearly.

Aplicação

Hydroxyphenyl fluorescein solution is used to detect highly reactive oxygen species (hROS). It is a suitable probe to detect cellular death caused by bacterial antibiotics.
Fluorescent reagent for the detection of highly reactive oxygen species (hROS). Fluorescence intensity increases after the immediate reaction with hROS such as hydroxyl radical and peroxynitrite. Since HPF does not react with nitric oxide, superoxide and hydrogen peroxide, it can differentially detect peroxynitrite. HPF is resistant to light-induced autoxidation.

Características e benefícios

The following are the benefits of Hydroxyphenyl fluorescein solution:

  • HPF is resistant to light-induced autooxidation.
  • Hypochlorite(-OCl) does not react with HPF.
  • HPF does not react with nitric oxide, superoxide, and hydrogen peroxide. 
  • HPF has low reactivity with HOCl.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

135.5 °F - (Dimethylformamide)

Ponto de fulgor (°C)

57.5 °C - (Dimethylformamide)

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

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Ken-ichi Setsukinai et al.
The Journal of biological chemistry, 278(5), 3170-3175 (2002-11-07)
We designed and synthesized 2-[6-(4'-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (HPF) and 2- [6-(4'-amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (APF) as novel fluorescence probes to detect selectively highly reactive oxygen species (hROS) such as hydroxyl radical (*OH) and reactive intermediates of peroxidase. Although HPF and APF themselves scarcely

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