69890

MES

BioUltra, ≥99.5% (T)

• Sinônimo(s): Ácido 2-(N-morfolino) etanossulfônico, Ácido 4-morfolino etanossulfônico
• Fórmula empírica (Notação de Hill): C₆H₁₃NO₄S · xH₂O
• Número CAS: 1266615-59-1
• Peso molecular: 195.24 (anhydrous basis)
• Número MDL: MFCD00149409
• Código UNSPSC: 12161700
• eCl@ss: 32129211
• ID de substância PubChem: 57652220
• NACRES: NA.25

Propriedades

• linha de produto: BioUltra
• Nível de qualidade: 200
• Ensaio: ≥99.5% (T)
• forma: powder or crystals
• Impurezas: insoluble matter, passes filter test
• resíduo de ignição (900 °C): ≤0.05%
• pH: 2.5-4.0 (25 °C, 0.5 M in H₂O)
• faixa de pH útil: 5.5-6.7
• pKa: 6.1
• solubilidade: H₂O: 0.5 M at 20 °C, clear, colorless
• traços de ânion: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• traços de cátion: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤20 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• Absorção UV: λ: 260 nm A_max: 0.025; λ: 280 nm A_max: 0.020
• cadeia de caracteres SMILES: O.OS(=O)(=O)CCN1CCOCC1
• InChI: 1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
• chave InChI: MIIIXQJBDGSIKL-UHFFFAOYSA-N

Descrição

Descrição geral

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Nota de preparo

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Outras notas

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.

Easily compare specifications for MES hydrate products with the MES hydrate specification table.

Biological buffer; useful pH-range 5.8-6.5; Mobile phase component in brain tubulin isolation by amino-activated gel chromatography; Buffer in plant tissue culture.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves