69889

MES

BioUltra, for molecular biology, ≥99.5% (T)

• Sinônimo(s): Ácido 2-(N-morfolino)etanossulfônico, Ácido 4-morfolino-etanossulfônico
• Fórmula linear: C6H13NO4S · H2O
• Número CAS: 145224-94-8
• Peso molecular: 213.25
• Beilstein: 6350956
• Número CE: 224-632-3
• Número MDL: MFCD00149409
• Código UNSPSC: 12161700
• eCl@ss: 32129211
• ID de substância PubChem: 329762028
• NACRES: NA.25

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• linha de produto: BioUltra
• Ensaio: ≥99.5% (T)
• forma: powder or crystals
• Impurezas: DNases, none detected; Insoluble matter, passes filter test; Phosphatases, none detected; Proteases, none detected; RNases, none detected
• resíduo de ignição (900 °C): ≤0.05% (as SO₄)
• pH: 2.5-4.0 (25 °C, 0.5 M in H₂O)
• faixa de pH útil: 5.5-6.7
• pKa (25 °C): 6.1
• pf: >300 °C (lit.)
• solubilidade: H₂O: 0.5 M at 20 °C, clear, colorless
• densidade: 10.66 g/mL
• traços de ânion: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• traços de cátion: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤20 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• Absorção UV: λ: 260 nm A_max: 0.025; λ: 280 nm A_max: 0.020
• cadeia de caracteres SMILES: O.OS(=O)(=O)CCN1CCOCC1
• adequação: suitable for DNase I test; suitable for Western blot; suitable for molecular biology
• aplicação(ões): clinical research; diagnostic assay manufacturing; life science and biopharma
• atividade externa: DNase, none detected; NICKase, none detected; RNase, none detected; protease, none detected
• InChI: 1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
• chave InChI: MIIIXQJBDGSIKL-UHFFFAOYSA-N

Descrição

Descrição geral

MES Buffer, known as one of the "Good′s" buffers designed for biological applications, plays a pivotal role in various aspects of research. This zwitterionic, morpholinic buffer is particularly valuable for its buffering capabilities within a pH range of 5.5 to 6.7. MES monohydrate, finds extensive utility in maintaining a stable environment in solutions used for cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. Additionally, MES stands out as an excellent choice for capillary electrochromatography, offering low ionic mobility. It serves as a safer alternative to toxic agents like cacodylate and non-zwitterionic buffers such as citrate and malate. While MES excels in buffering, it′s important to note that it is not recommended for use at pH 7.4, and alternative buffers should be explored in such cases. MES Monohydrate is also used to fine-tune the pH of growth media, stabilize enzymatic solutions, and as a component of PAGE running buffer in various experimental settings.

Aplicação

MES Monohydrate has been used:

• In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplasts using a haemocytometer
• As a component of Murashige and Skoog basal salt media
• In the preparation of total ionic strength adjustment buffer (TISAB)
• as a constituent in mobile phase solutions employed for the analysis of high molecular weight species and charge-related variants of biopharmaceutical proteins through SEC, HIC, and IEX chromatographic techniques
  

Características e benefícios

• Suitable as a Buffer component, for Electrophoresis and Molecular Biology
• Effective Buffering from pH 2.5-4.0 (25 °C, 0.5 M in H₂O) with a pKa of 6.1 (25 °C)
• Free from DNase, NICKase, RNase, and Protease

Nota de preparo

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Outras notas

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8C for months.
Sterilization: Sterilization should be by filteration through 0.2 uM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.

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Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves