47671

Formamida

BioUltra, for molecular biology, ≥99.5% (T)

• Sinônimo(s): Amida C₁, Amida fórmica
• Fórmula linear: HCONH₂
• Número CAS: 75-12-7
• Peso molecular: 45.04
• Beilstein: 505995
• Número CE: 200-842-0
• Número MDL: MFCD00007941
• Código UNSPSC: 12352111
• ID de substância PubChem: 57651075
• NACRES: NA.25

Propriedades

• grau: Molecular Biology; for molecular biology
• densidade de vapor: 1.55 (vs air)
• pressão de vapor: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• linha de produto: BioUltra
• Ensaio: ≥99.5% (T)
• Formulário: liquid
• temperatura de autoignição: 932 °F
• técnica(s): FISH: suitable; hybridization: suitable; protein extraction: suitable
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• resíduo de ignição: ≤0.1%
• índice de refração: n20/D 1.447 (lit.); n20/D 1.447
• pH: 7-9 (25 °C, 1 M in H₂O)
• p.e.: 210 °C (lit.)
• pf: 2-3 °C (lit.)
• solubilidade: H₂O: 10 M at 20 °C, clear, colorless
• densidade: 1.134 g/mL at 25 °C (lit.)
• traços de ânion: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• traços de cátion: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤1000 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• cadeia de caracteres SMILES: NC=O
• λ: 10 M in H₂O
• Absorção UV: λ: 260 nm A_max: ≤0.4; λ: 280 nm A_max: ≤0.1
• aplicação(ões): genomic analysis
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• chave InChI: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Descrição

Descrição geral

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

Aplicação

A formamida desestabiliza as duplas de ácidos nucleicos e pode ser usada, tipicamente, em uma concentração de 50% em protocolos de hibridização que requerem temperatura de hibridização mais baixas.

Formamide has been used:

• as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
• in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
• in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
• to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
• as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

Ações bioquímicas/fisiológicas

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

Características e benefícios

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Outras notas

For additional information on our range of Biochemicals, please complete this form.

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H351,H360D,H373
• Declarações de precaução: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Órgãos-alvo: Blood
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): 305.6 °F
• Ponto de fulgor (°C): 152 °C
• Equipamento de proteção individual: Eyeshields, Gloves, type ABEK (EN14387) respirator filter