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14533

Sigma-Aldrich

bisBenzimide H 33342 trihydrochloride

for fluorescence, ≥97.0% (HPLC)

Sinônimo(s):

2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

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About This Item

Fórmula empírica (Notação de Hill):
C27H28N6O · 3HCl · xH2O
Número CAS:
875756-97-1
Peso molecular:
561.93 (anhydrous basis)
Beilstein:
1234011
Número CE:
245-691-1
Número MDL:
MFCD00012678
Código UNSPSC:
12161505
ID de substância PubChem:
57647173
NACRES:
NA.25

grau

for fluorescence

Nível de qualidade

200

Ensaio

≥97.0% (HPLC)

fluorescência

λex 340 nm; λem 510 nm
λex 355 nm; λem 465 nm in TE buffer; DNA

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6

InChI

1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H

chave InChI

FYEVKHPLBHLWHK-UHFFFAOYSA-N

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Aplicação

bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Ações bioquímicas/fisiológicas

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Embalagem

Bottomless glass bottle. Contents are inside inserted fused cone.

Outras notas

Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Pyronin Y for microscopy (Bot., Fl., Hist.)

Sigma-Aldrich

83200

Pyronin Y

Iodeto de propídio ≥94% (HPLC)

Sigma-Aldrich

81845

Iodeto de propídio

Pyronin Y certified by the Biological Stain Commission, Dye content 50 %

Sigma-Aldrich

213519

Pyronin Y

DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

Millipore

Millipore

WBAVDFL01

Immobilon® Block - FL (bloqueador para detecção fluorescente)

M J Lydon et al.
Journal of cellular physiology, 102(2), 175-181 (1980-02-01)
A procedure has been investigated for sorting viable cells according to their DNA content. Cells are stained with the U.V. activated fluorochromes 4'6-diamidino-2-phenylindole (DAPI), Hoechst 33258 or Hoechst 33342, and sorted with a Fluorescence Activated Cell Sorter. Hoechst 33342 is
W M Grogan et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(6), 738-746 (1981-06-01)
The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells
M E Lalande et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(1), 363-367 (1981-01-01)
Flow cytometric analysis of the uptake of the DNA-specific and fluorescent probe Hoechst 33342 (HO342) offers a simple and rapid method for measuring membrane transport rates in mammalian cells and identifying cellular subpopulations that differ in their membrane transport rates.
Steven S Welc et al.
Nature communications, 10(1), 2788-2788 (2019-06-28)
Many potentially therapeutic molecules have been identified for treating Duchenne muscular dystrophy. However, targeting those molecules only to sites of active pathology is an obstacle to their clinical use. Because dystrophic muscles become extensively inflamed, we tested whether expressing a therapeutic
Ganna Aleshcheva et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(6), 2303-2314 (2015-02-15)
Real and simulated microgravity induce a variety of changes in human cells. Most importantly, changes in the cytoskeleton have been noted, and studies on microtubules have shown that they are gravisensitive. This study focuses on the effects of short-term real
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