382061

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride

Membrane-permeable, adenine-thymine-specific fluorescent stain.

• Sinônimo(s): Bisbenzimide H 33258 Fluorochrome, Trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
• Fórmula empírica (Notação de Hill): C₂₅H₂₄N₆O · 3HCl
• Número CAS: 23491-45-4
• Peso molecular: 533.88
• Número MDL: MFCD00012679
• Código UNSPSC: 12352111
• NACRES: NA.21

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥98% (HPLC)
• forma: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: yellow-green
• fluorescência: λ_ex 346 nm; λ_em 460 nm
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H
• chave InChI: OKQAASMPIOSOEX-UHFFFAOYSA-N

Descrição

Descrição geral

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Membrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
Adenine-thymine-specific fluorescent stain

Product does not compete with ATP.

Reversible: no

Advertência

Toxicity: Carcinogenic / Teratogenic (D)

Nota de preparo

Precipitates in phosphate buffered solutions

Reconstituição

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.

Outras notas

Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
Tarshis, M., et al. 1994. J. Immunol. Methods168, 245.
Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41.
Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939.
Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188.
Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable