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09978

Sigma-Aldrich

Sulfato de amônio

BioUltra, ≥99.0% (T)

Sinônimo(s):

Mascagnita

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About This Item

Fórmula linear:
(NH4)2SO4
Número CAS:
Peso molecular:
132.14
Número CE:
Número MDL:
Código UNSPSC:
12352314
eCl@ss:
38030408
ID de substância PubChem:
NACRES:
NA.26

linha de produto

BioUltra

Nível de qualidade

Ensaio

≥99.0% (T)

forma

crystals

Impurezas

insoluble matter, passes filter test

resíduo de ignição

≤0.01% (as SO4)

perda

≤0.1% loss on drying, 110 °C

pH

5.0-6.0 (25 °C, 1 M in H2O)

pf

>280 °C (dec.) (lit.)

solubilidade

H2O: 1 M at 20 °C, clear, colorless

densidade

1.77 g/mL at 25 °C (lit.)

traços de ânion

chloride (Cl-): ≤5 mg/kg
nitrate (NO3-): ≤50 mg/kg
phosphate (PO43-): ≤5 mg/kg

traços de cátion

Al: ≤5 mg/kg
As: ≤0.2 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤50 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤2 mg/kg
Sr: ≤5 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

Absorção UV

λ: 260 nm Amax: ≤0.037
λ: 280 nm Amax: ≤0.030

cadeia de caracteres SMILES

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

chave InChI

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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Descrição geral

Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.

Aplicação

Ammonium sulfate has been used:
  • to prepare mobile phase A for hydrophobic interaction chromatography (HIC)
  • as reference material for the thermal decomposition of NH4NO3 (S1–S6) thermal decomposition to N2O (S1-N2O–S6-N2O)
  • to prepare the stock solution for M63 medium
  • as a reagent in molecular biology and chromatography
  • in the precipitation and fractionation of proteins
  • in the purification of antibodies
  • for the crystallographic analysis of nucleic acids and proteins
  • in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography
  • for Sepharose chromatography of tRNAs using reverse salt gradients
  • in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis
  • HPLC analysis of phospholipids

Ações bioquímicas/fisiológicas

Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.

Outras notas

The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose chromatography of tRNAs using reverse salt gradients.

Informações legais

Sepharose is a trademark of Cytiva

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

T.D. Pollard
Methods in Enzymology, 85, 334-334 (1982)
Sepharose chromatography of transfer ribonucleic acids using reverse salt gradients.
G W Hatfield
Methods in enzymology, 59, 215-218 (1979-01-01)
Protein crystallization: the growth of large-scale single crystals.
G L Gilliland et al.
Methods in enzymology, 104, 370-381 (1984-01-01)
James F Hunter et al.
Environmental science & technology, 48(17), 10227-10234 (2014-08-06)
A large number of organic species emitted into the atmosphere contain cycloalkyl groups. While cyclic species are believed to be important secondary organic aerosol (SOA) precursors, the specific role of cyclic moieties (particularly for species with multiple or fused rings)
Kazunori Sugiura et al.
Biochemical and biophysical research communications, 457(3), 242-248 (2015-01-17)
Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004))

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