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Documentos Principais

S7545

Sigma-Aldrich

Acetato de sódio

BioXtra, ≥99.0%

Sinônimo(s):

Ácido acético

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About This Item

Fórmula linear:
CH3COONa
Número CAS:
Peso molecular:
82.03
Beilstein:
3595639
Número CE:
Número MDL:
Código UNSPSC:
12161700
eCl@ss:
39021906
ID de substância PubChem:
NACRES:
NA.25

linha de produto

BioXtra

Nível de qualidade

Ensaio

≥99.0%

Formulário

solid

temperatura de autoignição

1112 °F

Impurezas

≤0.0005% Phosphorus (P)
<0.01% Insoluble matter

pH

8.5-9.9 (25 °C, 246 g/L)

pKa 

4.76 (acetic acid)

pf

>300 °C (dec.) (lit.)

solubilidade

H2O: 1 M, clear, colorless

traços de ânion

chloride (Cl-): ≤0.002%
sulfate (SO42-): ≤0.003%

traços de cátion

Al: ≤0.0005%
Ca: ≤0.005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.03%
Mg: ≤0.0005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

cadeia de caracteres SMILES

[Na+].CC([O-])=O

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

chave InChI

VMHLLURERBWHNL-UHFFFAOYSA-M

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Descrição geral

Sodium acetate is commonly applicable in molecular biology experiments such as nucleic acid purification and precipitation, protein gel electrophoresis, protein crystallization, and high-performance liquid chromatography (HPLC). Sodium acetate is also used in plastic production, dyeing, and leather tanning.

Aplicação

Sodium acetate has been used:
  • in the isolation of DNA from bacterial samples of the human gastrointestinal tract
  • as a component of RNA elution buffer for the preparation of infrared (IR) fluorescent probes in northern blot technique
  • in the lysis buffer for cellular lysis to detect reversible oxidation of protein tyrosine phosphatases (PTPs) with the cysteinyl-labeling assay using IMR90 fibroblasts

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Journal of Molecular Liquids, 140(1-3), 10-14 (2008)
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Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern). Our method is as sensitive as other non-radioactive
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