71183

Acetato de sódio

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

• Sinônimo(s): Ácido acético
• Fórmula linear: CH₃COONa
• Número CAS: 127-09-3
• Peso molecular: 82.03
• Beilstein: 3595639
• Número CE: 204-823-8
• Número MDL: MFCD00012459
• Código UNSPSC: 12161700
• eCl@ss: 39021906
• ID de substância PubChem: 57652226
• NACRES: NA.25

Propriedades

• grau: for luminescence; for molecular biology
• Nível de qualidade: 200
• linha de produto: BioUltra
• Ensaio: ≥99.0% (NT)
• forma: solid
• temperatura de autoignição: 1112 °F
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• perda: ≤0.5% loss on drying, 150 °C
• pH: 8.5-10.0 (25 °C, 3 M in H₂O)
• pKa: 4.76 (acetic acid)
• pf: >300 °C (dec.) (lit.)
• solubilidade: H₂O: 3 M at 20 °C, clear, colorless
• traços de ânion: chloride (Cl^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• traços de cátion: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤10 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤10 mg/kg; Zn: ≤5 mg/kg
• cadeia de caracteres SMILES: [Na+].CC([O-])=O
• λ: 3 M in H₂O
• Absorção UV: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• chave InChI: VMHLLURERBWHNL-UHFFFAOYSA-M

Descrição

Aplicação

Sodium acetate is suitable for use as a buffer in:

• protein crystallization
• staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
• the preparation of standard (acetylcholine chloride) for hestrin assay
• substrate screening characterization for microfluidic microbial fuel cell array

Ações bioquímicas/fisiológicas

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Outras notas

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)