C1484

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin

≥95% purity (HPLC), solid

• Sinônimo(s): 1H-Pyrrole-2,5-dione, 1-(4-(7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl)phenyl)-, CPM
• Fórmula empírica (Notação de Hill): C₂₄H₂₂N₂O₄
• Número CAS: 76877-33-3
• Peso molecular: 402.44
• Número MDL: MFCD00077334
• Código UNSPSC: 12171500
• ID de substância PubChem: 24892413
• NACRES: NA.47

Propriedades

• Nome do produto: 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin, ≥95% (HPLC), solid
• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• Formulário: solid
• técnica(s): titration: suitable
• cor: yellow
• solubilidade: DMSO: soluble; methanol: soluble
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O
• InChI: 1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
• chave InChI: YGIABALXNBVHBX-UHFFFAOYSA-N

Descrição

Descrição geral

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM), is a highly fluorescent, sulfhydryl (-SH) group containing coumarin derivative. It has excitation an emission wavelengths of 355 and 460 nm, respectively.

Aplicação

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used:

• as a nontoxic substitute for conjugation with antibody(93)
• to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)
• in thermostability shift assay of recombinant protein(95)

Ações bioquímicas/fisiológicas

Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)