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95290

Sigma-Aldrich

Methyl Blue

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

Sinônimo(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Fórmula empírica (Notação de Hill):
C37H27N3Na2O9S3
Número CAS:
28983-56-4
Peso molecular:
799.80
Número do índice de cores:
42780
Número CE:
249-352-9
Número MDL:
MFCD00058509
Código UNSPSC:
12171500
ID de substância PubChem:
329770433
NACRES:
MA.02

grau

for microscopy (Bot., Hist.)
indicator (pH 9.4-14.0)

Nível de qualidade

200

forma

powder or crystals

técnica(s)

microbe id | staining: suitable

pH

9.4-14

solubilidade

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm
≥400 at 595-605 nm

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

chave InChI

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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Descrição geral

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Aplicação

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

>572.0 °F - Pensky-Martens closed cup

Ponto de fulgor (°C)

> 300 °C - Pensky-Martens closed cup

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análise (COA)

Lot/Batch Number
BCCB9353
BCBV2236
BCBR0621V
BCBM0641V
BCBH0469V

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