T9650

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

• Sinônimo(s): TAE buffer
• Número MDL: MFCD00236357
• Código UNSPSC: 41105319
• ID de substância PubChem: 329826908
• NACRES: NA.25

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• esterilidade: non-sterile; 0.2 μm filtered
• linha de produto: BioReagent
• forma: solution
• adequação: suitable for gel electrophoresis (after dilution to working concentration)
• atividade externa: Protease, none detected; RNAse, none detected
• cadeia de caracteres SMILES: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• chave InChI: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Descrição

Aplicação

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Outras notas

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Nota de preparo

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Warning
• Frases de perigo: H373
• Declarações de precaução: P260 - P313 - P501
• Classificações de perigo: STOT RE 2 Inhalation
• Órgãos-alvo: Respiratory Tract
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable