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93337

Sigma-Aldrich

Trizma® acetate

BioUltra, ≥99.0% (NT)

Sinônimo(s):

TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

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About This Item

Fórmula linear:
NH2C(CH2OH)3 · CH3COOH
Número CAS:
6850-28-8
Peso molecular:
181.19
Beilstein:
3702918
Número CE:
229-939-6
Número MDL:
MFCD00038951
Código UNSPSC:
12161700
ID de substância PubChem:
57653535
NACRES:
NA.25

linha de produto

BioUltra

Ensaio

≥99.0% (NT)

forma

crystalline powder

Impurezas

insoluble matter, passes filter test

resíduo de ignição

≤0.2%

perda

≤0.2% loss on drying, 20 °C (HV)

pH

6.0-7.0 (25 °C, 0.5 M in H2O)

solubilidade

H2O: 0.5 M at 20 °C, clear, colorless

traços de ânion

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

traços de cátion

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

Absorção UV

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.05

cadeia de caracteres SMILES

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

chave InChI

PIEPQKCYPFFYMG-UHFFFAOYSA-N

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Aplicação

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Outras notas

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding

Informações legais

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Chi-Lin Li et al.
The Analyst, 137(22), 5222-5228 (2012-10-04)
Oligonucleotide (T30695) modified gold nanoparticles (T30695-Au NPs) have been prepared and employed for quantification of lead ions (Pb(2+)) in blood. The detection of Pb(2+) ions is through the formation of Au-Pb alloys and oligonucleotide-Pb(2+) complexes that catalyze the H(2)O(2)-mediated oxidation
Monica Cubillos-Rojas et al.
Electrophoresis, 31(8), 1318-1321 (2010-03-24)
To separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3-15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris-acetate buffer (pH 7.0). Samples were prepared in a
Choice of buffer anion for the assay of adenosine 5'-triphosphate using firefly luciferase.
W W Nichols et al.
Analytical biochemistry, 114(2), 396-397 (1981-07-01)
M Ito et al.
Life sciences, 38(12), 1089-1096 (1986-03-24)
[3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when
Monica Cubillos-Rojas et al.
Methods in molecular biology (Clifton, N.J.), 869, 205-213 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show
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