89640

Toluidine Blue

8.74% (ZN (THEORY)), for microscopy (Hist., Vit.)

• Sinônimo(s): Tolonium chloride; Basic Blue 17
• Fórmula empírica (Notação de Hill): C₁₅H₁₆ClN₃S · 0.5ZnCl₂
• Número CAS: 6586-04-5
• Peso molecular: 373.97
• Número do índice de cores: 52040
• Beilstein: 4068052
• Número MDL: MFCD03548293
• Código UNSPSC: 12171500
• ID de substância PubChem: 329769757
• NACRES: MA.02

Propriedades

• grau: for microscopy (Hist., Vit.)
• Nível de qualidade: 200
• Ensaio: 8.74% (ZN (THEORY))
• Formulário: powder or crystals
• técnica(s): microbe id | staining: suitable
• cor: green to very dark green
• solubilidade: methanol: 0.01 g/10 mL, blue to very deep blue
• εmax: ≥800 at 621-636 nm in water
• adequação: suitable for microscopy
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
• InChI: 1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
• chave InChI: DAQXTSXITPUGRG-UHFFFAOYSA-L

Descrição

Descrição geral

Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.

Aplicação

Toluidine Blue is suitable for use in the following applications:

1. to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
2. in metachromatic staining of sulfated mucins and related materials
3. as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
4. staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Ações bioquímicas/fisiológicas

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)