B8438
Alcian Blue solution
1% in 3% acetic acid, pH 2.5
Sinônimo(s):
Alcian Blue 8XG solution
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About This Item
Produtos recomendados
forma
liquid
Nível de qualidade
concentração
1% in 3% acetic acid, pH 2.5
cor
dark blue to very dark blue
faixa de pH
2.4-2.6
Amax
0.45-0.60 (1:400 in H2O)
aplicação(ões)
diagnostic assay manufacturing
hematology
histology
temperatura de armazenamento
room temp
Aplicação
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
Ações bioquímicas/fisiológicas
Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Stain Technology, 51(2), 131-134 (1976)
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
The Biochemical Journal, 131(2), 351-357 (1973)
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